Biography of Col. Ellison Onizuka, Challenger Astronaut

Memoir of Col. Ellison Onizuka, Challenger Astronaut At the point when the space transport Challenger detonated on January 28, 1986, the disaster ended the lives of seven space explorers. Among them was Col. Ellison Onizuka, an Air Force veteran and Nasa space explorer who turned into the main Asian-American to travel to space. Quick Facts: Ellison Onizuka Conceived: June 24, 1946 in Kaelakekua, Kona, HawaiiDied: January 28, 1986 in Cape Canaveral, FloridaParents: Masamitsu and Mitsue OnizukaSpouse: Lorna Leiko Yoshida (m. 1969)Children: Janelle Onizuka-Gillilan, Darien Lei Shuzue Onizuka-MorganEducation: Bachelors and Masters degrees in Aerospace Engineering from the University of Colorado Career: Air Force pilot, NASA AstronautFamous Quote: Your vision isn't restricted by what your eyes can see, however by what your brain can envision. Numerous things that you underestimate were viewed as ridiculous dreams by past ages. On the off chance that you acknowledge these past achievements as ordinary, at that point think about the new skylines that you can investigate. From your vantage point, your instruction and creative mind will convey you to places which we won’t accept conceivable. Make an incredible most and the world will be a superior spot since you attempted. On the mass of the Hawaii Challenger Center. Early Life Ellison Onizuka was brought into the world under the name Onizuka Shoji in Kaleakekua, close Kona, on the Big Island of Hawaii, on June 24, 1946. His folks were Masamitsu and Mitsue Onizuka. He grew up with two sisters and a sibling, and was an individual from Future Farmers of America and the Boy Scouts. He went to Konawaena High School and regularly discussed how he would dream about flying out to the stars that he could see from his home on the island.â Training Onizuka left Hawaii to consider designing at the University of Colorado, accepting a single men degree in June 1969 and a bosses degree a couple of months after the fact. That equivalent year he additionally wedded Lorna Leiko Yoshida. The Onizukas had two girls: Janelle Onizuka-Gillilan and Darien Lei Shizue Onizuka-Morgan.â After graduation, Onizuka joined the United States Air Force and filled in as a flight test architect and aircraft tester. He likewise centered around frameworks security building for various planes. During his flying vocation, Onizuka increased in excess of 1,700 flight hours. While in the Air Force, he prepared at the Flight Test Center at Edwards Air Force Base in California. While piling on flying time and testing planes for the Air Force, he additionally dealt with frameworks for various trial military aircraft.â Onizukas NASA Career <img information srcset=https://www.thoughtco.com/thmb/v2edD4OL0wsWVmgAMGZS1Gs7xqo=/300x0/filters:no_upscale():max_bytes(150000):strip_icc()/1024px-STS-51-C_crew-5c678ea646e0fb0001f933e4.jpg 300w, https://www.thoughtco.com/thmb/1cVWy99QLHlGRqqbO2emHaJ1ZMI=/481x0/filters:no_upscale():max_bytes(150000):strip_icc()/1024px-STS-51-C_crew-5c678ea646e0fb0001f933e4.jpg 481w, https://www.thoughtco.com/thmb/XVBRSIUOBuwl_A55cJdN2CCn02M=/662x0/filters:no_upscale():max_bytes(150000):strip_icc()/1024px-STS-51-C_crew-5c678ea646e0fb0001f933e4.jpg 662w, https://www.thoughtco.com/thmb/bK87AAovnE-w3rGvTvDmGqHmcjQ=/1024x0/filters:no_upscale():max_bytes(150000):strip_icc()/1024px-STS-51-C_crew-5c678ea646e0fb0001f933e4.jpg 1024w information src=https://www.thoughtco.com/thmb/HylqEi8A28j5TnVhDd9_GQLd42Y=/1024x819/filters:no_upscale():max_bytes(150000):strip_icc()/1024px-STS-51-C_crew-5c678ea646e0fb0001f933e4.jpg src=//:0 alt=Crew of STS 51C, including Ellison Onizuka. class=lazyload information click-tracked=true information img-lightbox=true information expand=300 id=mntl-sc-square image_1-0-12 information following container=true /> The team relegated to the STS-51C strategic (bowing in front left to right) Loren J. Schriver, pilot; and Thomas K. Mattingly, II, officer. Standing, left to right, are Gary E. Payton, payload expert; and strategic James F. Buchli, and Ellison L. Onizuka. Propelled on board the Space Shuttle Discovery on January 24, 1985 at 2:50:00 pm (EST), the STS-51C was the primary strategic to the Department of Defense (DOD).  NASA Ellison Onizuka was chosen as a NASA space traveler in 1978 and left the Air Force with a position of lieutenant colonel. At NASA, he took a shot at the bus flight joining lab group, strategic, and, while in space, overseeing payloads on circle. He took his first trip on STS 51-C on board the van Discovery in 1985. It was a top-mystery trip to dispatch a payload from the Department of Defense, the principal ordered crucial the orbiters. That flight additionally proclaimed another first by making Onizuka the principal Asian-American to fly in space. The flight went on for 48 circles, allowing Onizuka 74 hours on circle. <img information srcset=https://www.thoughtco.com/thmb/fSLubVOx9hOEcs8UGt5bp1cdXBM=/300x0/filters:no_upscale():max_bytes(150000):strip_icc()/Onizuka_in_flight-5c678b36c9e77c0001675983.jpg 300w, https://www.thoughtco.com/thmb/SHYNAyGlvO92n2w4L8gZp3chluk=/481x0/filters:no_upscale():max_bytes(150000):strip_icc()/Onizuka_in_flight-5c678b36c9e77c0001675983.jpg 481w, https://www.thoughtco.com/thmb/bqOiQm1Bf0fcRripdvRqFE1HBDc=/662x0/filters:no_upscale():max_bytes(150000):strip_icc()/Onizuka_in_flight-5c678b36c9e77c0001675983.jpg 662w, https://www.thoughtco.com/thmb/uwv4iaiaeAAbEFOIJXxYC7LC_t0=/1024x0/filters:no_upscale():max_bytes(150000):strip_icc()/Onizuka_in_flight-5c678b36c9e77c0001675983.jpg 1024w information src=https://www.thoughtco.com/thmb/RUm4t547HIdFHT6wJQt_goOq23I=/1024x667/filters:no_upscale():max_bytes(150000):strip_icc()/Onizuka_in_flight-5c678b36c9e77c0001675983.jpg src=//:0 alt=Ellison Onizuka (left) in trip during his first transport crucial. class=lazyload information click-tracked=true information img-lightbox=true information expand=300 id=mntl-sc-square image_1-0-15 information following container=true /> Ellison Onizuka (left) in trip with Loren Shriver, during his first transport crucial.  NASA Onizukas Final Mission His next task was on STS 51-L, set to dispatch Challenger into space in January 1986. For that flight, Onizuka was appointed strategic obligations. He was joined by educator in-space selectee Christa McAuliffe, Gregory Jarvis, Ronald McNair, Michael J. Smith, Judith Resnik, and Dick Scobee. It would have been his subsequent trip to space. Shockingly, Col. Onizuka died alongside his crewmates when the rocket was crushed during a blast 73 seconds after dispatch. <img information srcset=https://www.thoughtco.com/thmb/x4fwgyX2fklV-agbXSPhdhbugRw=/300x0/filters:no_upscale():max_bytes(150000):strip_icc()/sharon-christa-mcauliffe-ronald-emcnair-gregory-jarvis-ellison-onizuka-michael-jsmith-francis-rscobee-judith-aresnik-50597660-5c757f55c9e77c0001d19bf5.jpg 300w, https://www.thoughtco.com/thmb/y9Sbm4Lr-pXg-QMyl-8rKPhUEqo=/724x0/filters:no_upscale():max_bytes(150000):strip_icc()/sharon-christa-mcauliffe-ronald-emcnair-gregory-jarvis-ellison-onizuka-michael-jsmith-francis-rscobee-judith-aresnik-50597660-5c757f55c9e77c0001d19bf5.jpg 724w, https://www.thoughtco.com/thmb/ecEITqjGTLvn65vFuHwXZby5AOM=/1148x0/filters:no_upscale():max_bytes(150000):strip_icc()/sharon-christa-mcauliffe-ronald-emcnair-gregory-jarvis-ellison-onizuka-michael-jsmith-francis-rscobee-judith-aresnik-50597660-5c757f55c9e77c0001d19bf5.jpg 1148w, https://www.thoughtco.com/thmb/LPkM-UGTHACEgJQqqkQy0Rz1qLs=/1997x0/filters:no_upscale():max_bytes(150000):strip_icc()/sharon-christa-mcauliffe- ronald-emcnair-gregory-jarvis-ellison-onizuka-michael-jsmith-francis-rscobee-judith-aresnik-50597660-5c757f55c9e77c0001d19bf5.jpg 1997w information src=https://www.thoughtco.com/thmb/LqB6Q71IdUwcrc-K8kIoIcHUWiE=/1997x1649/filters:no_upscale():max_bytes(150000):strip_icc()/sharon-christa-mcauliffe-ronald-emcnair-gregory-jarvis-ellison-onizuka-michael-jsmith-francis-rscobee-judith-aresnik-50597660-5c757f55c9e77c0001d19bf5.jpg src=//:0 alt=Sharon Christa Mcauliffe;Ronald E. Mcnair;Gregory Jarvis;Ellison Onizuka;Michael J. Smith;Francis R. Scobee;Judith A. Resnik class=lazyload information click-tracked=true information img-lightbox=true information expand=300 id=mntl-sc-square image_1-0-19 information following container=true /> Team of Space Shuttle Challenger X (L-R first line) space explorers Smith, Scobee, McNair (L-R back) Onizuka, payload authority/educator McAuliffe, payload spec. Jarvis space explorer Resnik, Johnson Space Center. The LIFE Picture Collection/Getty Images/Getty Images Respects and Legacy The vast majority at NASA who worked with him recall Colonel Onizuka as a pilgrim. He was a man with an incredible comical inclination, and somebody who regularly energized individuals, especially youthful understudies to utilize their creative mind and insight as they sought after their professions. During his short vocation, he was granted the Air Force Commendation Medal, Air Force Outstanding Unit Award, and the National Defense Service Medal. After his passing, Col. Onizuka was respected in an assortment of ways, including the Congressional Space Medal of Honor. He was raised to the position of Colonel in the Air Force, a respect given to the individuals who lose their lives in administration. Col. Onizuka is covered at the National Memorial Cemetery of the Pacific in Honolulu. His achievements have been memorialized on structures, lanes, a space rock, a Star Trek shuttlecraft, and other science and designing related structures. Different foundations, including the Gemini Observatories and different offices in Hawaii, hold yearly Ellison Onizuka days for building and science symposia. The Challenger Center Hawaii keeps up a salute to his support of his nation and to NASA. One of two air terminals on the Big Island is named for him: the Ellison Onizuka Kona International Airport at Keahole. Space experts additionally perceive his administration with the Onizuka Center for International Astronomy. It

The merchant of venic Essays - British Films, English-language Films

The trader of venic The Merchant of Venice by WIlliam Shakespeare. The Merchant of Venice is a play about retribution, equity, deseption and companionship. The story is about, Shylock a riches Jew, loaning one of his adversaries, Antonoi, 3,000 ducats. In spite of the fact that Antonio is a rich vendor every one of his assets are in his boats, exchanging too far off nations, but since he needs to assist his friend,Bassanio, he must choose the option to approach Shylock for an advance , not to realize it could prompt his passing. In this play there is a major contrast between the Christian charaters and Shylock, the primary distinction being Shylock is by all accounts progressively keen on his cash and business instead of human connections, which the Christian charaters appear to b e increasingly intrigued by. In any case, in one piece of the play Shylock is by all accounts hurt at the reality his little girl has sold a ring, which his perishes spouse gave him, for a monkey. (Act 3 Scene 1 ) It was my turquoise, I had it off Leah when I was a lone ranger. I Would not have given it for a Wild of monkeys. This demonstrates a side to Shylock that we have never observed. It shows the adoration he had and still has for his perished spouse Leah. Shylock shows how harmed and double-crossed he feels that his little girl has sold his ring by saying that he wouldn't had parted with it for a wild of monkeys . Another subject in the play that Shylock brings out is predjudice. He makes a decision about individuals before he allows them to show what they are truly like. He has diverse veiws on various characters depanding on what their identity is. ( Act 1 Scene 3 ) I cap him for he is a Christian This shows how he feels against antonio , a Christian whom he has never met and it shows how partiality he can be. In one piece of the play Shylock demonstrates a side to him we have never observed. Rather if considering him to be the man who is eager and egotistical, we see a man who has been derided and abuseed. ( Act 1 Scene 3 ) Numerous a thime and oft in the ralto You have evaluated me about my cash Also, my usanes still I have borne it With a patient shrug, for fortitude Is the identification of all our clan. You Consider me a skeptic cut-throught Canine and spit on mu Jewish Gabardine. THe statement shows that shylock feels nearly hurt at the reality the Christian characters are looking donw on him and hir religion in spite of the fact that he was doing precisely the same to Bassanio. Taking everything into account we perceive how the dramatist utilizes charaterisation to uncover the topics of the play. I think Shaspeare shows this well overall and the play was energizing and charming to peruse. The Character of Shylock in Merchant of Venice Scarcely any characters made by Shakespeare exemplifies unadulterated shrewdness like the character of Shylock in The Merchant of Venice. Shylock is a usurer and a malicious, murderous elderly person overwhelmed by plotting the defeat of his foes. He is a malignant,vengeful character, overwhelmed by venomous malice1; an image of unfeeling, unmitigated villainy, hard of hearing to each intrigue of humanity2. Shylock is the opponent inverse the innocent, basically great Antonio, the hero; who must shield himself against the fiend Shylock. The underhanded he speaks to is one reason Shakespeare decided to describe Shylock as a Jew, as Jews of his time were viewed as the offspring of the Devil, the crucifiers of Christ and difficult rejecters of God's insight and Christianity. Be that as it may, when Shakespeare made Shylock, he didn't embed him in as a simply level character, expended uniquely with the villainy of his plot. One of the incredible abilities that Shakespeare had, comments Shakespeare expert Harrold R. Walley, was his capacity to make each key character act like a genuine, balanced individual. Walley said of the entirety of Shakespeare's characters, legend or scalawag, that Their lead is constantly introduced as legitimate and reasonable from their place of view3. To keep up the artistic trustworthiness of the play, Shakespeare is under the need

English Class Essay Topics

English Class Essay TopicsThere are a couple of English class exposition points that will in general be rehashed in different subjects. These are typically extremely essential and simple subjects which you can use to begin on the things that you have to know to traverse your course.History is one of the most significant subject that you can use as a theme for your exposition. You can utilize it to see history when all is said in done and the political circumstance around the globe. You can likewise utilize it to investigate specific times of history, or to take a gander at why history has occurred as it has. The article that you compose ought to have the option to take a gander at the significance of the things that occurred in history and how they have influenced current society.Other subjects to be utilized as a significant piece of the paper are religion and folklore. They can be utilized to see how societies identify with one another and furthermore to take a gander at how religi on is utilized in the public arena. It can assist you with seeing the significance of religion when you are attempting to comprehend the way that cutting edge society operates.Literature is one of the more well known subjects to use for your paper points. You can utilize it to see what has been expounded on specific issues and occasions in the public eye and to see what is critical to expound on. You can likewise utilize it to take a gander at what the different works of writing state about specific times of history or about the world as a whole.A extraordinary subject to utilize is science. It very well may be utilized to talk about the significance of science and innovation and how they have assisted with forming present day society. Science can likewise be utilized to take a gander at how science can change the way that society capacities. You can likewise utilize it to take a gander at how science is a significant effect on the manners in which that society conducts itself.Anoth er point that will in general be utilized very oftenas exposition themes is religion. This is typically a result of the way that religion is such a focal subject in our general public and it is something that is frequently taken a gander at from a logical perspective. There are various parts of religion and one of the most significant parts is the way that individuals identify with their God.Sports is additionally an exceptionally mainstream subject to use in the articles that you compose. The games that are shrouded in this sort of article are normally ones that you know about. You can likewise see what sports have intended to society and how they are utilized to assist with molding our general public. You can likewise take a gander at how they sway the colleagues just as the players and how this can influence individuals and what they do regarding the two games and the remainder of society.There are numerous themes that you can use for your English class paper subjects. You can ut ilize subjects, for example, history, writing, religion, science, and sports. You will find that these are themes that have been secured previously and they can assist you with rounding out the subject regions that you have to finish on your classwork.

Impact of Glucocorticoids on the C2C12 cells’ Differentiation

Question: Discuss about the Impact of Glucocorticoids on the C2C12 cells Differentiation and Proliferation. Answer: Rationale All the vertebrates have three main types of muscles that can be differentiated on the basis of their morphology and their roles in the body. The skeletal muscles enable the vertebrates to move from one geographical area to the other. Smooth muscles are lined with internal organs like blood supplying vessels. The third one is the cardiac muscle that is located within the heart. Its main role is to help in pumping of the blood thought the body thus enabling metabolic processes. Skeletal muscles account 40% of the body weight. The skeletal muscle cells are the largest cells in the body. During the developmental stages the myoblast also know as precursor fuses to form a multinucleated skeletal muscle cells through a process known as myogenesis. Its possible to study Myogenesis outside the body of the host using cell lines myoblast like C2C12 cell lines. C2C12 cells are easily proliferate under more concentrated serum conditions and differentiate but fusion can only take place under low serum state. Therefore, making it a good tool in tests that involve myogenesis, muscle biology and metabolism. The cell line starts to show myogenesis when it riches 100% confluency. But when transferred to differentiation medium, the cells quickly increase myogenesis and differentiation results to the formation of myotubes (Ricotti et al 2012) Results The experiment started by setting up 2 well plates and allowed to proliferate for about 4 days and then allowed to differentiate for about 10 days. These were 2 well plates out of six. The remaining 4 well plates were set by the laboratory demonstrators. Two of the remaining well plates that contained C2C12 were seeded on the day 1 before of the Lab# 3. The other two wells in the culture containing C2C12 were seeded on day 4 and they represented Day 4-time point. The proliferation extent was accessed by comparing the C2C12 cells seed on day and day 4 (figure 2). In the case of more cells in day 4 than day 1 proved that the proliferation might have occurred. Differentiation was to be accessed by comparing the day 1 cell and day 4. In case there is different cells, that shows that the differentiation took place. During 2-4 labs, effect of dexamethasone on C2C12 cell proliferation was evaluated. A control experiment was set for day1,2 and 14 (Figure 3) and each treated with dexamethasone. In order to access the effect of the dexamethasone on cell line proliferation, 100uM dexamethasone was incubated one of the 2-day 4 cell well plates. The number of cells in the control day 4 well was compared with the experiment treated day 4 well. The number of cell that were present in each of the cell wells of all the cultured plates during differentiation may affect the extent to which differentiation is attained. If a big number of cells recorded in a well at the beginning of differentiation stage, that shows the interaction of cells to form myotubes. Cell viability Proliferated and differentiated cells of C2C12 were then fixed using 10% neutral buffered formalin. Then they were stained using eosin and hematoxylin. The morphologies of these cells were then accessed by counting cell on the hemocytometer. The following cell count were observed from the secondary square of hemocytometer. These were the cells that took up the trypan blue in their cytoplasm and nucleus. From the outer corner of the hemocytometer, 7 cells were observed at the top and 3cells on top left. The secondary square at the outer border of the hemocytometer grid, the following number of cells were observed. Square# 2 had 4 clear cells, 6 blue cells. Square #3 1 clear cell, 2 blue cells. Square #4 3clear cells and 4clear cells. Using immunocytochemistry to access C2C12 cell proteins The main objective of the lab was to study how to do immunocytochemistry on proliferation and differentiation of C2C12. Protein Proliferation cell nuclear antigen (PCNA) was also analyzed and the Myosin heavy chain (MHC) on the 3rd and 7th day. Glucocorticoid receptors localization in control and DMSO were assessed on the 3rd day. Immunocytochemistry was performed on fixed C2C12 cells from the 3rd and 7th day. Proliferation and differentiation was assessed on the two days consecutively in the experimental procedures described in 2nd and 3rd lab. One dish of the cells was fluorescently labeled for myosin heavy chain (MHC), Glucocorticoid or PCNA using secondary and primary antibodies. PCNA and (MHC) was assessed on third and seventh day using C2C12. Protein expression differed between differentiation and Proliferation of C2C12 Cells. Glucocorticoid was assessed on the 3rd day in control and C2C12 cell treated with dexamethasone. C2C12 cells treated with the dexamethasone were put insi de dexamethasone for a period of 24 hours before fixed on the day 3. The time period allowed for dexamethasone initiated glucocorticoid receptor and its transfer to the nucleus. Discussion To our own understanding, this is the first demonstration does not only induce proliferation and differentiation. Dexamethasone also causes hypertrophy in muscles depending on the differentiation stage at the point of application. The effect caused by dexamethasone is step-dependent. Dexamethasone observed at myoblast cause hypertrophy in muscles and enhances myotube differentiation as well. Similar case has been observed when the myotube were treated using dexamethasone one of the glucocorticoids. The main objective this Lab test was to investigate the effect that would be caused by glucocorticoid in differentiation and proliferation processes of C2C12 cells. More emphasis focused on the dexamethasone which demonstrated the genes that were involved in the proliferation and differentiation. A vital thing that was investigated in the experiment was C2C12 cells with dexamethasone, PCNA and myosin heavy chain. This treatment aimed at the growth stimulation of the cells as well as to accelerate differentiation process. (Figure 1,2,3) shows that treating C2C12 cell with dexamethasone resulted to a significant high number of C2C12 cells proliferation. This observation is in agreement with what was recorded by (D'Angelo et al 2012) who described C2C12 cell growth and differentiation stimulation with glucocorticoids producing almost similar results. Again (Fujimoto et al 2012) s howed the impact of glucocorticoid on the proliferation and differentiation of C2C12 cells. Some of the results obtained at different points of the day, showed that differentiation can be accelerated significantly when exposed to dexamethasone. The same results have been illustrated by (Goljanek et al 2012) in the experiment done to show the pivotal for C2C12 cells myoblast differentiation. The hemocytometer was used in the counting of the cells according to (Riss et al 2016). The secondary square on the hemocytometer has got about 9 squares. Each of those square measure around one mm squared. The central part is the counting region on the hemocytometer. Only those cells lined on the two lines of the big square are suppost to be counted (Shenoy et al 2014). According to (Minetti et al 2014) the suspension must be diluted enough to prevent counting some of the cells a second time due to overlap. The purpose of using Trypan Blue was to differentiate between the blue cells (dead cells) from the clear cells (live cells). The staining method is also called the dye exclusion staining. The counting of cells done provided us with a % visibility cells of 40.74% and the cell density of 375000cell/ml (Figure5). The data was obtained using the light microscope where the magnification technique was to be applied in order to reach the desired cell type (Huri et al 2014). The cells in the chosen square were counted so that the total number of cells required was to be a significant statistical count (Alberts et al 2013). Discussion Questions. Myogenic index is termed as the number of nuclei that are contained in the cells that have more than nuclei divided by the total number of cell nuclei provided in the image taken. These images are then used to solve the myogenic index by finding the percentage number of nuclei in the myotubes using Jenner-Giemsa culture. The culture should be put in the differentiation media and incubated for five days. Giemsa G25 (Sigma Aldrich) is used to fix the cells and then three different photos are captured. The nuclei in the myotube and those in the cells are then counted in each photo. Myogenic index is then calculated by dividing the total number of nuclei in the cell by total number of nuclei in the myotubes. Myogenesis is the process being determined. Increase in the myogenic index shows accuracy in the experimental images whereby several nuclei are captured (Srikuea et al 2012) C6orf32 gene is gene found in chromosome 6 and its expression multiples during the differentiation of the human fetal myoblast. It is expressed both in non- myogenic and primary cells and is separated from the 18th week of human fetal development. Experiments on c2c12 murine myoblast cell revealed that cells without c60rf32 shows a myogenic differentiation defect while when it is expressed in excess, it accelerates the formation of filopodia. This gene plays a number of functioning like filopodia formation, cytoskeletal arrangement and promotion of myogenic cell differentiation. The gene c6orf32 is specialized to specific functions whereby the cells lacking this gene are unable to perform those functions and thus results to serial defects (Thrivikraman et al 2013) Immunochemistry experimental design can be used in visualization of specific proteins. In the cells. In the experimental process, two antibodies are used in the targeting of a particular protein. These two antibodies are the secondary antibody and primary antibody. Secondary antibodies with fluorophore binds on the target and binds to the primary antibodies. Therefore, when one is performing immunochemistry, its important to use correct fixative. A secondary antibody must bind to primary antibodies other than non-specific antigen. Serum contains an array of the antibodies. Hence it should be blocked because it might block the binding of secondary and primary antibodies (Shi et al 2013). References D'Angelo, M. A., Gomez-Cavazos, J. S., Mei, A., Lackner, D. H., Hetzer, M. W. (2012). A change in nuclear pore complex composition regulates cell differentiation. Developmental cell, 22(2), 446-458. Fujimoto, Y., Abematsu, M., Falk, A., Tsujimura, K., Sanosaka, T., Juliandi, B., ... Nakashima, K. (2012). Treatment of a mouse model of spinal cord injury by transplantation of human induced pluripotent stem cell?derived long?term self?renewing neuroepithelial?like stem cells. Stem Cells, 30(6), 1163-1173. Goljanek-Whysall, K., Pais, H., Rathjen, T., Sweetman, D., Dalmay, T., Mnsterberg, A. (2012). Regulation of multiple target genes by miR-1 and miR-206 is pivotal for C2C12 myoblast differentiation. J Cell Sci, 125(15), 3590-3600. Ricotti, L., Polini, A., Genchi, G. G., Ciofani, G., Iandolo, D., Vazao, H., ... Pisignano, D. (2012). Proliferation and skeletal myotube formation capability of C2C12 and H9c2 cells on isotropic and anisotropic electrospun nanofibrous PHB scaffolds. Biomedical Materials, 7(3), 035010 Srikuea, R., Zhang, X., Park-Sarge, O. K., Esser, K. A. (2012). VDR and CYP27B1 are expressed in C2C12 cells and regenerating skeletal muscle: potential role in suppression of myoblast proliferation. American Journal of Physiology-Cell Physiology, 303(4), C396-C405. Alberts, B., Bray, D., Hopkin, K., Johnson, A., Lewis, J., Raff, M., ... Walter, P. (2013). Essential cell biology. Garland Science. Thrivikraman, G., Mallik, P. K., Basu, B. (2013). Substrate conductivity dependent modulation of cell proliferation and differentiation in vitro. Biomaterials, 34(29), 7073-7085. Shi, K., Lu, J., Zhao, Y., Wang, L., Li, J., Qi, B., ... Ma, C. (2013). MicroRNA-214 suppresses osteogenic differentiation of C2C12 myoblast cells by targeting Osterix. Bone, 55(2), 487-494. Huri, P. Y., Wang, A., Spector, A. A., Grayson, W. L. (2014). Multistage adipose-derived stem cell myogenesis: an experimental and modeling study. Cellular and Molecular Bioengineering, 7(4), 497-509. Luo, W., Wu, H., Ye, Y., Li, Z., Hao, S., Kong, L., ... Zhang, X. (2014). The transient expression of miR-203 and its inhibiting effects on skeletal muscle cell proliferation and differentiation. Cell death disease, 5(7), e1347. Minetti, G. C., Feige, J. N., Bombard, F., Heier, A., Morvan, F., Nrnberg, B., ... Fornaro, M. (2014). Gi2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation. Molecular and cellular biology, 34(4), 619-630. Shenoy, A., Blelloch, R. H. (2014). Regulation of microRNA function in somatic stem cell proliferation and differentiation. Nature Reviews Molecular Cell Biology, 15(9), 565. Riss, T. L., Moravec, R. A., Niles, A. L., Duellman, S., Benink, H. A., Worzella, T. J., Minor, L. (2016). Cell viability assays. Madigan, M. T., Martinko, J. M., Parker, J. (2017). Brock biology of microorganisms (Vol. 13). Pearson.